Figure 3

Selectivity of RLSB binding to representative plastid-encoded transcripts of maize. Levels of each of the indicated plastid-encoded transcripts were analyzed using RNA immunopurification and real-time quantitative PCR (RIP/qRT-PCR), as described in the text. RNA was extracted from pellet fractions following immunoprecipitations from maize chloroplasts, using antisera against RLSB, or as controls, anti-PEPCase or no added antisera. qRT-PCR was performed using primers for each of the seven transcripts indicated. All mRNA levels were quantified relative to plastid-encoded transcripts of plastid ribosomal protein (rpl2). Results shown are averaged from 2 repeats of 3 independent experiments. The dotted line at 0.48 indicates the average level of amplification from the no-antibody control reactions; this was used as the background value. Statistical significance was calculated using Student’s t-test. For each bar, P values were less than 0.05.