Integration of promoters, inverted repeat sequences and proteomic data into a model for high silencing efficiency of coeliac disease related gliadins in bread wheat

Table 2 Prolamins and non-gluten contents, gliadins and glutenins contents, total protein and starch contents, SDS sedimentation test and 1000 grain weight of transgenics grouped by silencing fragment and promoter used

	iTarget	Gliadin (μg/mg of flour)				Glutenin (μg/mg of flour)				(μg/mg of flour)		Protein (%)	Starch (%)	SDSS	Weight 1000
	iTarget	ω	α	γ	Total	LMW	HMW	Total	Gli/Glu	Prolamins	Non-gluten	Protein (%)	Starch (%)	(mL · g − 1)	(g)
a	C	11.81a	34.61a	23.98c	74.22a	14.62c	9.62d	24.65ab	3.07a	97.45a	32.94a	14.09a	56.17a	12.33c	44.36a
	g	15.46a	41.81a	3.27a	61.21a	17.54a	11.51a	29.44a	2.08a	92.11a	35.56a	15.05a	55.13ab	13.02a	43.98a
	o	5.59b	8.86b	3.79a	18.00b	14.57b	18.31c	32.77ab	0.56b	50.83b	78.63b	14.59a	55.06bc	11.28bc	40.85b
	go	5.82b	7.11b	1.76b	14.20b	10.57b	13.98b	25.22b	0.59b	38.44b	98.36b	15.45b	53.68c	9.86b	38.06b
b		Gliadin				Glutenin			Gli/Glu	Prolamins	Non-gluten	Protein	Starch	SDSS	Weight 1000
	Promoter	ω	α	γ	Total	LMW	HMW	Total	Gli/Glu	Prolamins	Non-gluten	Protein	Starch	SDSS	Weight 1000
	D	1	0.74	0.15c	0.58	1.04a	1.62	1.26a	0.46	0.76a	1.00a	1.07	0.98	0.93a	0.90b
	G	0.84	0.65	0.07a	0.49	0.95b	1.63	1.22a	0.4	0.68ab	1.00ab	1.06	0.97	0.93a	0.94a
	GD	0.79	0.61	0.01b	0.44	0.65c	1.52	1.02b	0.43	0.60b	1.01b	1.11	0.96	0.75b	0.92ab

(a) iTarget, silencing fragments; g, γ-gliadin silencing fragment; o, ω/α-gliadin silencing fragment; go, both silencing fragments; ω, ω-gliadins; α, α-gliadins; γ, γ-gliadins; total, total gliadin content; HMW, high molecular weight; LMW, low molecular weight; Gli/Glu, ratio total gliadin content/total glutenin content; Protein, total protein content in percent of total dry flour weight; Starch, total starch content in percent of total dry flour weight; SDSS, SDS sedimentation test; Weight 1000, 1000 kernel weight in grams. (b) Promoter, the promoter used to drive the silencing fragments; C, control line; G, γ-gliadin promoter; D, H-hordein promoter; GD, the γ-gliadin and D-hordein promoter; the values in the promoter table are means of the normalized values relative to control lines (transgenic value/control value). Values within the same column followed by the same letter are not significantly different at P < 0.05 multiple comparison of means test. Variables with values without a letter are not significantly different.

ISSN: 1471-2229