Figure 1From: The effect of Translationally Controlled Tumour Protein (TCTP) on programmed cell death in plantsEffect of TCTP on BAX induced cell death. Cell death quantification of N. benthamiana leaf discs transiently over-expressing Arabidopsis thaliana TCTP (AtTCTP) and/or BAX. A shows ion leakage calculated relative to time point T0 in response to 2 μM dexamethasone (DEX), which induced expression of BAX. Statistical significances were calculated using analysis of variance (P < 0.05) and are indicated as follows: a BAX vs. AtTCTP, b AtTCTP + BAX vs. AtTCTP, c AtTCTP + BAX vs. BAX. B shows western blot analyses of leaf discs expressing AtTCTP over time (following time points are shown: 2, 21, 27, 45, 54, and 69 hours of disc floating); AtTCTP is shown without (upper panel) and with (lower panel) co-expression of BAX. C shows tobacco leaf discs expressing AtTCTP, BAX, or both while floating on 2 μM DEX containing solution for 69 hours (panel on the left side). Panels on the right side show trypan blue staining of dead cells from leaf discs expressing BAX and/or AtTCTP as indicated. D shows control experiments: relative ion leakage measurements in response to expression of ΔC-BAX, StrepII-tag, or co-expression of ΔC-BAX + AtTCTP in response to 2 μM DEX. No statistically significant differences were calculated using analysis of variance.Back to article page