Effects on JA content and JA biosynthetic genes in OsNAP transgenic lines and WT plants. A, Endogenous levels of JA in OsNAP transgenic and WT plants. Leaf samples of OsNAP-overexpressing transgenic lines (line 15 and line 17), OsNAP RNAi lines (RNAi-1 and RNAi-2) and WT plants at seedling and mature stage were used for the analysis. Each point represents a mean (three replicates) ± SD. n.d., no detected. B, qRT-PCR analysis for JA biosynthetic genes in leaves of the OsNAP-overexpressing lines (line 17 and line 19) and WT plants. Transcripts associated with leaves of OsNAP-overexpressing lines and WT plants at the seedling stage were analyzed. C, qRT-PCR analysis for JA biosynthetic genes in leaves of the OsNAP RNAi lines and WT plants at the mature stage. The first-strand cDNAs were prepared using RNAs extracted from the leaves of OsNAP RNAi lines and WT plants at the mature stage. Bars represent mean (3 replicates) ± standard deviation. *P < 0.05, **P < 0.01 (t test).