Figure 7From: Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescenceEffects on JA content and JA biosynthetic genes in OsNAP transgenic lines and WT plants. A, Endogenous levels of JA in OsNAP transgenic and WT plants. Leaf samples of OsNAP-overexpressing transgenic lines (line 15 and line 17), OsNAP RNAi lines (RNAi-1 and RNAi-2) and WT plants at seedling and mature stage were used for the analysis. Each point represents a mean (three replicates) ± SD. n.d., no detected. B, qRT-PCR analysis for JA biosynthetic genes in leaves of the OsNAP-overexpressing lines (line 17 and line 19) and WT plants. Transcripts associated with leaves of OsNAP-overexpressing lines and WT plants at the seedling stage were analyzed. C, qRT-PCR analysis for JA biosynthetic genes in leaves of the OsNAP RNAi lines and WT plants at the mature stage. The first-strand cDNAs were prepared using RNAs extracted from the leaves of OsNAP RNAi lines and WT plants at the mature stage. Bars represent mean (3 replicates) ± standard deviation. *P < 0.05, **P < 0.01 (t test).Back to article page