Figure 4

OsNAP overexpression lines showed an accelerated leaf senescence phenotype. A, Expression level of OsNAP in the OsNAP overexpression lines and wild-type plants at the seedling stage. B and C, Photographs of flag leaves in OsNAP overexpression lines and WT plants at 30 (B) and 40 DAH (C). D, Expression level of OsNAP in the OsNAP overexpression lines and wild-type plants at the grain-filling stage. The first-strand cDNAs were prepared using RNAs extracted from the flag leaves of OsNAP overexpression lines and WT plants at the grain-filling stage. Each value represents the mean ± SD evaluated from three replicates. E and F, Time course determinations of chlorophyll content and net photosynthetic rate in flag leaves of OsNAP overexpression lines and WT plants after heading. The values are means (± SD) of four replicates.