OsMPK20-4 and OsMPK3 have role in plant defense. (A) Both OsMPK20-4 and OsMPK3 give resistance against the disease. Tobacco leaves infiltrated with mock (IM only), Agrobacterium strain GV3101, OsMPK20-4-HA and OsMPK3-Myc in 1:1 ratio were challenged with P. syringae pv tabaci (OD600 0.1) infection after 24 hours of agroinfiltration. Photographs were taken after 72 hours of infection. (B) OsMPK20-4 and OsMPK3 play synergistic role in plant defense. OsMPK20-4-HA and OsMPK3-Myc induced resistance was determined according to the schematic representation (upper panel), and the growth of P. syringae pv tabaci was plotted as log cfu/leaf disc (lower panel). Three independent experiments were conducted and the representative figure is shown. t test P values: * < 0.05, ** < 0.01. (C) OsMPK20-4 and OsMPK3 interaction plays role in stomatal defense. Upper panel shows the localization of the shown proteins before P. syringae pv tabaci infection. Lower panel shows the sudden translocation of both the rice MAPKs into the nuclei and stomatal guard cells after 24 h of infection. The pictures were taken from confocal laser scanning microscope (Leica AOBS system). Bars = 20 μm.