Figure 4

Quantitative analyses across three separate spaceflight experiments. Quantitative RT-qPCR analyses were used to check the consistency of gene expression in root RNA across three separate TAGES flight experiments: Run 1B, Run 2A, and Run 2B. Runs 1B, 2A, and 2B were composed of identical Arabidopsis lines and grown under identical conditions, although in three distinct windows of time on the ISS (see Methods). The fold-change between flight and ground control is presented for five genes showing statistically significant (p < 0.01) differential expression in the microarrays conducted for the Run 2B plants (black). The RT-qPCR fold-change values (in log_[2]) for each gene are presented below the array values in gradients of gray bars: Run 2B (dark gray), Run 1B (medium gray), Run 2A (light gray). Error bars for each RT-qPCR set indicate Standard Deviation with an n = 3 of biological replicates. The asterisks (***) associated with array data bars indicate p ≤0.005; otherwise, p ≤0.01. The public ID numbers for the genes presented are: DDF1 (At1g12610), DREB2A (At5g05410), TCH4 (At5g57560), JAZ7 (At2g34600), ELIP1 (At3g22840).