Figure 5

The expression of three maize genes associated with the defense response PR1, PRms and WIP1 was measured using semi-quantitative PCR in three mutant two wild type seedlings derived from the cross Tx303 × Rp1-D21 -H95. GAPDH and EF1α were included as reference genes. RNA was extracted from the plants grown at a constant 30°C for three weeks, the temperature was then dropped to a constant 18°C for seven days and RNA was extracted from leaves of the same plants.