Figure 3

Subcellular distribution of ascorbate and glutathione in chloroplasts of Col-0. Transmission electron micrographs showing the subcellular distribution of ascorbate (a) and glutathione (b) in chloroplasts of Arabidopsis thaliana Col-0 plants, exposed to light intensities of 1,500 μmol m^-2 s^-1. Gold particles bound to ascorbate and glutathione could be found in different densities within chloroplast (C), mitochondria (M), nuclei (N), and the cytosol but not in cell walls (CW). Inside chloroplasts of control plants ascorbate and glutathione were detected in the stroma and inside the lumen of thylakoids (arrows). Bars = 0.5 μm.