Figure 4
From: The dual targeting ability of type II NAD(P)H dehydrogenases arose early in land plant evolution
Subcellular localizations of Arabidopsis and rice NDB type proteins. GFP was fused to the C-terminus of NDB1, NDB2 and NDB4 from Arabidopsis thaliana (At) and NDB1 and NDB2 from Oryza sativa (Os). Additionally, the last 10 amino acids were fused at the C-terminus of GFP. Subcellular targeting was analyzed in both Arabidopsis cell suspension or onion epidermal tissue along with RFP tagged mitochondrial or peroxisomal controls. The protein accession number, the location of GFP and the number of amino acids used are shown for each construct. Mitochondria (M) and peroxisomes (Px) are indicated. M-RFP - mitochondrial RFP, Px-RFP - peroxisomal RFP. Scale bar indicates 10 μm.