Figure 4

Cross sections of non-adventitious roots from control, non-flooded-plants (A,C) adventitious roots from flooded seedlings (B,D). The Safranin O and Fast Green FCF sections show starch accumulation in the cortical cells at 0.5 cm from the root tip (A,B). These sections were mounted on slides and examined under the light microscope. Immunolocalication of PIP1aquaporins (C,D) was carried out with sections taken at 0.5 cm from the root tip. The sections were examined under the fluorescence microscope (Leica DMRXA Upright Microscope) with a green light filter I3 at 450–490 nm excitation and 510 nm emission. Epidermis (EP), Endodermis (EN) and xylem (XYL) structures are indicated with arrows.