RT-PCR analysis using the primers 5′UTR-exon F and uidA 200 R/ gfp R in (A) transgenic Gladiolus leaves (B) transgenic Arabidopsis leaves, and (C) transiently transformed tobacco leaves. Western blot analysis of GFP shown in bottom panel of C. The vectors were pUC-GUS for Gladiolus, pCAMBIA for Arabidopsis, and pBI121 for tobacco. pCAMBIA contains “LacZ alpha, truncated” between the GUBQ1promoter and uidA gene”. Abbreviations: Non-transformed plants (N), G1-1 (1), G1-3 (3), G1-1 promoter with act7 intron [1(A)], transient expression with pBI121 vector (−). Actin gene primers specific to each plant species analyzed were used as the reference gene to verify RT-PCR performance and the possibility of DNA contamination (bottom panel in A and B, middle panel in C).