Protein accumulation pattern of seeds from the cross of B73 x P6z1CRNAi. Lanes 1–3, vitreous kernels with genotype B73/-; −/−; lanes 4–6, opaque kernels with genotype B73/-; P6z1RNAi/-. Zein and non-zein accumulation patterns of B73/-; −/− and B73/-; P6z1RNAi/- were analyzed by 15% SDS-PAGE. Protein from 500 μg of maize flour was loaded in each lane. M, protein markers from top to bottom being 250, 150, 100, 75, 50, 37, 25, 20, 15 and 10 kDa. The size of each zein band is indicated with numbers in the “kDa” column.