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Table 1 Comparison of the N-terminal amino acid sequences derived from protein sequencing with those deduced from the cloned DNA sequences encoding for HMW-GS in three Aegilops species

From: Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding

Species Subunit Residue
1 2 3 4 5 6 7 8 9 10 11 12
Triticum aestivum x-type consensus E G E A S G/E Q L Q C E R/H
Ae. bicornis 1Sbx sequenced E G E A S G Q L Q C E R
1Sbx deduced E G E A S G Q L Q C E R
Ae. longissima 1Slx sequenced E G E A S G Q L Q C   
1Slx deduced E G E A S G Q L Q C E R
Ae. sharonensis 1Sshx sequenced E G E A S G Q L Q C E  
1Sshx deduced E G E A S G Q L Q C E R
T. aestivum y-type consensus E G E A S R/K Q L Q C E R
Ae. bicornis 1Sby sequenced E G E A S R Q L Q C E R
1Sby deduced E G E A S R Q L Q C E R
Ae. longissima 1Sly sequenced E G E A S R Q L Q C E  
1Sly deduced E G E A S R Q L Q C E R
Ae. sharonensis 1Sshy sequenced E G E A S R Q L Q C E R
  1Sshy deduced E G E A S R Q L Q C E R
  1. The consensus sequences of x-type and y-type HMW subunits of bread wheat were also list as standard. The results indicated that the amino acid sequences translated from the cloned genes are perfectly matched to those of the native protein of seeds.