Figure 3
Golgi dictyosomes and chloroplasts distribution in Physcomitrella patens protonemata. Bright field and confocal images of 5 distinct zones in caulonemata (A) and chloronemata (B) expressing the YFP-Man fusion protein to visualize Golgi dictyosomes (Golgi). The chloroplasts (Chlorop) are visualized by chlorophyll autofluorescence. The top panel shows the bright field (BF). Green and red signals represent YFP and chlorophyll autofluorescence respectively. Bottom panels show the merged image of the green and red signals. Images are displayed as maximal projections of confocal sections. Arrow and arrowhead show respectively small area deprived of Golgi dictyosomes and dividing chloroplasts. Scale bar 10 μm.