Figure 2

Expression analysis of starch biosynthetic genes in developing endosperm and leaves. RNA expression was analyzed by quantitative PCR on cDNA from developing bread wheat endosperm and leaves from parent lines (955 and 981), SBEIIa mutant lines (060 and 109) and wild-type sibling lines (248 and 250). Fold expression is relative to that observed in an SBEIIa mutant line. Two independent lines were evaluated in triplicate for (A) SBEIIa expression, (B) SBEIIb expression, (C) SSI expression, and (D) GBSSI expression in endosperm tissue; and for (E) SBEIIa expression and (F) SSI expression in leaves. SBEIIb and GBSSI expression were not detectable in leaves. Error bars represent SEM.