Figure 4
Analysis of ABA levels in corolla and their effect on BA emission. (A) ABA levels were quantified by LC-MS/MS in isolated corollas from flowers of WT and ir-hd20 plants grown either under control or salt stress conditions. (B) BA was trapped from individual flowers from 3 pm to 9 am during the first night of corolla opening. WT and ir-hd20 plants were grown either under control or salt stress conditions (BA levels in control conditions correspond to the data showed in Figure 3a and were included here for comparison). (C) Flowers from WT plants were sprayed either with 1 mL of solvent or with 1 mL a solution containing ABA (1 μg mL-1) at 8 pm. BA was trapped from individual flowers from 10 pm to 9 am and analyzed by GC-MS. BA levels are expressed as detailed in legend of Figure 3. (D) Flowers from WT plants were clipped off at the base of the pedicle and placed inside a microcentrifuge tube containing either 100 μL of water or 100 μL ABA (1 μg mL-1). Emitted BA was analyzed in real time with a zNOSETM. Asterisks correspond to P < 0.05 (Student’s t-test; ir-hd20 vs. WT levels; n = 6; bars denote ± SE).