Interaction of AtClpC2 and AtClpD with TPs located at the N-terminal end of GST. GST, TP-GST or TP1234-GST were used to load the gluthathione-agarose resin. Blots show the remaining AtClpC2 (A) or AtClpD (C) after each wash. Coomassie blue-stained SDS-PAGE gels below each blot show the integrity of GST and GST fusion proteins along the washing steps. Digitized bands from six different blots were quantified using the software GelPro. Graphs show the percentage of residual AtClpC2 (B) or AtClpD (D, lower band; E, upper band) in the TP-GST- (squares), the TP1234-GST- (diamonds), or in the GST-containing resin (circles) after each washing step.