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Table 2 Somatic chromosome number, meiotic configurations and pollen stainability in F 1, F 2 and OP plants

From: Experimental evidence for the ancestry of allotetraploid Trifolium repens and creation of synthetic forms with value for plant breeding

Genotype

Somatic chromosome number

Number of PMC

Meiotic configurations

Anaphase I disjunction

Pollen stainability

   

I

II

III

IV

  
   

x ¯ (range)

x ¯ (range)

x ¯ (range)

x ¯ (range)

  

440–1

2n=2x=16

>300

0 (0)

8 (0)

0 (0)

0 (0)

8–8

5%

88–01

2n=4x=32

80

0 (0)

8 (6–10)

0 (0)

4 (3–5)

16–16

78%

440–1 OP-3

2n=4x=32

70

0 (0)

8 (6–14)

0 (0)

4 (1–6)

16–16

18%

440–1 OP-9

2n=4x=32

55

0 (0)

9 (8–14)

0 (0)

3.5 (1–4)

16–16

27%

440–1 OP- 55

2n=4x=32

63

0 (0)

8.2 (6–12)

0 (0)

3.9 (2–5)

16–16

53%

440–1 OP-88

2n=4x+1=33

13

2.2 (1–5)

8.1 (2–14)

0.85 (0–2)

3.0 (0–5)

1617

43%

440–1 OP-153

2n=3x=24

43

2.0 (1–4)

2.6 (1–5)

5.6 (4–7)

0 (0)

12–12*

11%

F2 (2x)†

2n=2x=16

475

0 (0)

8 (0)

0 (0)

0 (0)

88

 
  1. * two cells showed 1113 disjunction and approximately 10 showed lagging chromosomes at anaphase I.
  2. seven verified 2x plants produced from open pollination of 440–1. Two cells from one plant had 7 II and 2 I.