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Table 2 Somatic chromosome number, meiotic configurations and pollen stainability in F 1, F 2 and OP plants

From: Experimental evidence for the ancestry of allotetraploid Trifolium repens and creation of synthetic forms with value for plant breeding

Genotype Somatic chromosome number Number of PMC Meiotic configurations Anaphase I disjunction Pollen stainability
    I II III IV   
    x ¯ (range) x ¯ (range) x ¯ (range) x ¯ (range)   
440–1 2n=2x=16 >300 0 (0) 8 (0) 0 (0) 0 (0) 8–8 5%
88–01 2n=4x=32 80 0 (0) 8 (6–10) 0 (0) 4 (3–5) 16–16 78%
440–1 OP-3 2n=4x=32 70 0 (0) 8 (6–14) 0 (0) 4 (1–6) 16–16 18%
440–1 OP-9 2n=4x=32 55 0 (0) 9 (8–14) 0 (0) 3.5 (1–4) 16–16 27%
440–1 OP- 55 2n=4x=32 63 0 (0) 8.2 (6–12) 0 (0) 3.9 (2–5) 16–16 53%
440–1 OP-88 2n=4x+1=33 13 2.2 (1–5) 8.1 (2–14) 0.85 (0–2) 3.0 (0–5) 1617 43%
440–1 OP-153 2n=3x=24 43 2.0 (1–4) 2.6 (1–5) 5.6 (4–7) 0 (0) 12–12* 11%
F2 (2x) 2n=2x=16 475 0 (0) 8 (0) 0 (0) 0 (0) 88  
  1. * two cells showed 1113 disjunction and approximately 10 showed lagging chromosomes at anaphase I.
  2. seven verified 2x plants produced from open pollination of 440–1. Two cells from one plant had 7 II and 2 I.