Figure 3

Cytosine methylation changes in a fragment of Tos17 (encompassing portions of the 5'-LTR- and body-region) in the S0 generation of the etoposide-treated plants of Nipponbare, determined by bisulfite sequencing. Nine and 10 clones were arbitrarily sequenced for the control and the 10 mg/L etoposide-treated plants, respectively. All three types of cytosines, CG (red circles), CHG (blue circles) and CHH (green circles) were shown in the map. Filled and empty circles denote methylated and unmethylated cytosines, respectively. The red, blue and green columns in the histograms refer to the collective methylation levels (in percentage) respectively of CG, CHG and CHH, of the LTR- and body-regions. The nucleotide sequence of this analyzed fragment was presented in Additional file 1. De novo methylation at six CHH sites as a result of the etoposide-treatment were arrowed.