Changes in DNA methylation in the S0 generation of etoposide- treated plants of genotypes Nipponbare and 93-11 relative to their respective wild-type control plants. Each genotype was treated with two concentrations of etoposide, 10 mg/L and 20 mg/L, respectively. “Control” represents plants treated with ddH2O. DNA methylation changes occurred only at the CHG context of the 5’-CCGG sites (in MspI digestion, denoted by rectangles), and the changes occurred only in Nipponbare. Note that the two etoposide concentrations showed the same changes. The Elongation factor gene is intrinsically unmethylated in the rice genome and thus showed a single monomorphic band among all treated plants in both HpaII and MspI digestions of each genotype. This monomorphic hybridizing pattern of Elongation factor also validated complete digestion by the methylation-sensitive isoschizomers in all DNA samples.