Figure 2

Workflow for the sequential extraction of PB proteins. The PB extract purified on iodixanol gradient was resuspended and incubated in a Laëmmli buffer without reductant (solubilization buffer 1) to extract soluble and membrane proteins. After centrifugation, the supernatant (S) was collected and the pellet was resuspended in a complete Laëmmli buffer (solubilization buffer 2) to extract proteins linked through disulfide bridges. The supernatant (R) was collected and the pellet was resuspended in a Laëmmli buffer and heated at 95°C for 5 min to solubilize the PB core. The supernatant was collected (H).