Figure 5
Validation of array data by comparing the expression estimates from NimbleGen wheat microarrays and quantitative reverse transcription PCR for differentially expressed genes. (A) - (H): Differentially expressed E3 ligase genes. (I) - (O): Differentially expressed hormone-related genes. Blue graphs represent DNA microarray data depicting the expression intensity of each transcript (left y-axis); red graphs depict quantitative reverse transcription RT-PCR results (right y-axis representing the relative expression level) during 11 developmental stages (x-axis representing the thermal time after anthesis (°Cdays)). The correlation coefficient (R) between the two graphs is indicated for each gene. Values for the two curves represent the ratio between the value for each stage and the 40°Cday value.