Figure 2
Expression of lactoferrin in seven T 8 transgenic wheat lines determined by Northern blot (A) and Western blot (B) analyses using two top leaves and inflorescences at growth stage Feekes 10.5. Northern blot: Total RNA from transgenic and control plants were hybridized with a 32P-labeled lactoferrin cDNA probe. 18s RNA was used as a loading control. Western blot: Immunodetection of lactoferrin protein in transgenic wheat plants using a polyclonal antibody reagent. Lane PC, purified lactoferrin protein; lane C, control wheat plant, lane 1-7 transgenic wheat lines: BLFW 119, 351, 378, 424, 685, 892 and 1102. Position of 85 kDa molecular weight marker is shown with an arrow on the right.