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Figure 9 | BMC Plant Biology

Figure 9

From: Identification of five B-type response regulators as members of a multistep phosphorelay system interacting with histidine-containing phosphotransfer partners of Populus osmosensor

Figure 9

Expression analysis of RR12, 13, 14, 16, 19 by RT-PCR. RNAs isolated from roots (R), stems (S), petioles (P) and leaves (L) under control (c) or stressed (s) conditions, were reverse transcribed and used as template for PCR amplification. PCR reactions were performed using B-type RRs specific primers under optimal conditions for each primer set (A: 30 cycles, B: 40 cycles). Expression profile of a housekeeping gene, Clathrin (CLA), was realized with 25 cycles of PCR amplification.

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