Table 2 Tomato orthologues of candidate regulatory genes for L-ascorbic acid metabolism
Gene | Reference Gene inArabidopsis | Unigene | Name | F-primer (5′-3′) | R-primer (5′-3′) |
|---|---|---|---|---|---|
L-galactose pathway | |||||
GDP-mannose pyrophosphorylase | At2g39770 | SGN-U568548 | SlGMP1 | CAGGTAGCATTATCGGTTGG | TTGATCTCCTTGTGGGGTAA |
SGN-U568547 | SlGMP2 | GTGTAGTTTTGCCCCACAAG | AGGAGAACTGGAAACCAACC | ||
SGN-U584300 | SlGMP3 | AGAAGCGACTGGGAGAGTTG | GAATCCGGTCCAAAACAGAA | ||
GDP-mannose-3,5-epimerase | At5g28840 | SGN-U581327 | SlGME1 | TTCCTGTCCAACACATTCCT | CTTTCTCGATCTGCTCCTTG |
SGN-U580362 | SlGME2 | ATTCGAGATGTGGGGAGATG | GGAAGGTTCTTGCCATCAAA | ||
GDP-L-galactose phosphorylase | At4g26850 | SGN-U579800 | SlGGP1 | TCCAAGTGTCGTTGCCATAA | TCGGAAGTAAGGGTTTGCTG |
SGN-U573852 | SlGGP2 | GCTCTCCATTTTGCCAGAGA | CCCCTTCCTTGCCAGTATTT | ||
L-galactose-1-P-phosphatase | At3g02870 | SGN-U600217 | SlGPP1 | TGGTGGCGCTGTAATAGTGA | AACGCTTCCTTGAGATGAGG |
SGN-U568299 | SlGPP2 | AGGTCCCTTCGTATGTGTGG | GAACCCTCCAGCTTCTTTCA | ||
L-galactose dehydrogenase | At4g33670 | SGN-U565558 | SlGalDH | AGTAGCAACGACTGGAATGG | TGCACAACAGGAGATCACAA |
L-galactonolactone dehydrogenase | At3g47930 | SGN-U585649 | SlGLDH | AGATTGAGGTTCCCAAGGAC | TTAGATAGGATGCGGTTTGG |
AsA recycling pathway | |||||
Monodehydroascorbate reductase | At1g63940 | SGN-U583672 | SlMDHAR1 | CAAGGGTTTCGGTTCCTTCT | CTGCATTTCCTCCTCCAACT |
At3g52880 | SGN-U573751 | SlMDHAR2 | AGATCGTTGGTGCATTCCTC | AAAACTGATGCCCTCCTGTG | |
At3g27820 | SGN-U584073 | SlMDHAR3 | AGGAATGGAATGTGCTGCTT | GACCGTGCCTTTCACAAACT | |
Dehydroascorbate reductase | At1g19570 | SGN-U583361 | SlDHAR1 | AGGTGGCTCTTGGACACTTC | CTTCAGCCTTGGTTTTCTGG |
At5g16710 | SGN-U577467 | SlDHAR2 | CACCCAGAGGGTTTTGCTTA | CTCCAGTGCCTGTGAGATGA | |