A diploid wheat TILLING resource for wheat functional genomics

BMC Plant Biology

Table 3 Estimated population size required to identify a truncation mutant in some TILLING populations* (Modified from Parry et al. 2009 [32])

Species	Ploidy	Frequency of mutation	Population size used	Population size required	Reference
Arabidopsis	diploid	1/300 kb	3 072	18 000	Greene et al. 2003 [20]
	diploid	1/89 kb	3 712	5 340	Martin et al. 2009 [34]
Sorghum	diploid	1/526 kb	768	31 560	Xin et al. 2008 [11]
Rice	diploid	1/294 kb	768	17 640	Till et al. 2006 [51]
	diploid	1/135 kb	767	8 100	Suzuki et al. 2008 [49]
Barley	diploid	1/1000 kb	9 216	60 000	Caldwell et al., 2004 [6]
	diploid	1/374 kb	4 906	22 440	Talame et al. 2008 [7]
Einkorn wheat	diploid	1/92 kb	1 532	5 520	This study
Durum wheat	tetraploid	1/40 kb	768	2 400	Slade et al., 2005 [12]
	tetraploid	1/51 kb^a	1 386	3 060	Uauy et al., 2009 [13]
Arabidopsis	tetraploid	<1/100 kb	528	6 000	UC Davis Genome Center
Bread wheat	hexaploid	1/24 kb	1 152	1 440	Slade et al., 2005 [12]
	hexaploid	1/23-38 kb^b	2 348	1 380	Dong et al., 2009 [25]
	hexaploid	1/38 kb^a	1 536	2 280	Uauy et al., 2009 [13]
Oat	hexaploid	1/20-40 kb	2 550	1 200	Chawade et al. 2010 [17]

*at 95 % probability in some TILLING populations, assuming 5% of the mutations being non-sense.
^anormalized to 50% G/C content.
^bdepending upon cultivar and EMS treatment.

ISSN: 1471-2229