Table 2 Nature of mutations found in the T. monococum TILLING population

COMT1_A

1-4-G6

C>T

Hetero

Mis-sense

Thr>Met

90

0.14

0

2-1-E6

G>A

Hetero

Silent

-

91

-

-

4-1-A3

G>A

Homo

Mis-sense

Ala>Lys

95

0.36

+1

4-1-A11

G>A

Hetero

Mis-sense

Glu>Lys

92

0.24

+1

5-7-D6

G>A

Hetero

Mis-sense

Ala>Thr

65

0.25

+4

6-10-G8

G>A

Homo

Mis-sense

Glu>Lys

116

0.23

+1

COMT1_B

1-2-B8

C>T

Hetero

Mis-sense

Ala>Val

275

0.45

+4

3-3-E4

G>A

Hetero

Silent

-

298

-

-

4-3-B10

G>A

Hetero

Mis-sense

Met>Ile

261

0.55

0

4-4-H5

G>A

Homo

Mis-sense

Glu>Lys

271

0.41

−1

5-8-A1

G>A

Hetero

Mis-sense

Glu>Met

298

0.16

−4

6-10-F3

C>T

Hetero

Mis-sense

Pro>Ser

248

0.42

+2

HCT2_A

1-3-B12

G>A

Hetero

Mis-sense

Gly>Arg

131

0.25

−2

2-5-G6

G>A

Hetero

Silent

-

100

-

-

5-8-C3

C>T

Homo

Silent

-

72

-

-

5-6-C11

G>A

Hetero

Silent

-

85

-

-

HCT2_B

1-1-A4

G>A

Hetero

Mis-sense

Glu>Lys

208

0.45

+1

1-2-E7

G>A

Homo

Silent

-

371

-

-

2-1-E12

C>T

Hetero

Silent

-

395

-

-

2-6-H8

G>A

Hetero

Mis-sense

Asp>Asn

330

0.56

−2

3-2-G8

C>T

Homo

Silent

-

359

-

-

3-2-F5

C>T

Hetero

Mis-sense

Thr>Met

314

0.19

−1

4-2-A9

G>A

Homo

Mis-sense

Ala>Thr

275

0.50

−3

4-1-B1

C>T

Hetero

Silent

-

308

-

-

4-3-G4

G>A

Hetero

Mis-sense

Val>Ile

310

0.59

+2

5-8-C8

C>T

Hetero

Mis-sense

Leu>Phe

267

0.37

+2

6-10-A8

G>A

Hetero

Silent

-

287

-

-

6-10-F11

G>A

Hetero

Silent

-

414

-

-

4CL1_B

2-1-H5

G>A

Hetero

Mis-sense

Met>Ile

405

0.31

−3

3-4-C10

C>T

Hetero

Mis-sense

Thr>Met

345

0.11

−3

4-1-E2

G>A

Hetero

Silent

-

340

-

-

5-8-A10

G>A

Homo

Mis-sense

Gly>Asp

370

0.28

−7

6-9-G10

G>A

Hetero

Non-sense

Trp>Stop

338

-

-

4CL1_C

1-2-D3

C>T

Hetero

Intronic

-

-

-

-

1-1-A7

G>A

Hetero

Intronic

-

-

-

-

3-4-B7

C>T

Hetero

Intronic

-

-

-

-

4-3-B6

C>T

Hetero

Intronic

-

-

-

-

5-7-E8

G>A

Homo

Mis-sense

Val>Ile

476

0.27

+1

5-6-B8

C>T

Hetero

Silent

Gly

485

-

-

6-9-H8

C>T

Hetero

Silent

Gly

534

-

-

W1

1-2-C5

G>A

Hetero

Mis-sense

Gly>Ser

95

0.20

−4

1-1-D7

C>T

Hetero

Mis-sense

Ala>Val

58

0.19

Not found**

2-1-H5

C>T

Hetero

Silent

-

104

-

-

2-5-E5

C>T

Hetero

Intronic

-

-

-

-

3-3-B3

G>A

Homo

Mis-sense

Gly>Glu

101

0.17

−1

3-3-E3

C>T

Hetero

Intronic

-

-

-

-

3-2-G3

C>T

Hetero

Silent

-

60

-

-

4-3-B5

G>A

Homo

Silent

-

51

-

-

4-4-F9

G>A

Hetero

Intronic

-

-

-

-

4-4-G9

G>A

Hetero

Mis-sense

Arg>His

153

0.09

+1

5-8-E6

C>T

Homo

Intronic

-

-

-

-

6-10-F1

C>T

Hetero

Intronic

-

-

-

-

6-9-H7

G>A

Hetero

Mis-sense

Glu>Lys

143

0.14

−1

DOS

1-4-D8

G>A

Hetero

Silent

-

253

-

-

1-2-G4

G>A

Hetero

Mis-sense

Glu>Lys

328

0.12

−2

2-5-D6

G>A

Hetero

Intronic

-

-

-

-

2-6-G4

G>A

Homo

Intronic

-

-

-

-

3-2-D1

G>A

Homo

Silent

-

359

-

-

3-3-G9

G>A

Hetero

Mis-sense

Val>Met

254

0.10

−6

3-4-H2

C>T

Hetero

Mis-sense

Ala>Val

305

0.13

−3

4-3-F4

G>A

Homo

Intronic

-

-

-

-

4-1-H2

G>A

Hetero

Silent

-

294

-

-

5-7-A8

C>T

Hetero

Mis-sense

Ala>Val

399

0.11

+2

5-7-D1

G>A

Hetero

Silent

-

315

-

-

5-5-H1

C>T

Hetero

Mis-sense

Ala>Val

252

0.10

+2

6-10-E6

G>A

Hetero

Silent

-

277

-

-

6-9-F6

C>T

Homo

Silent

-

346

-

-