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Table 2 Nature of mutations found in the T. monococum TILLING population

From: A diploid wheat TILLING resource for wheat functional genomics

Primer set (F+R) Plant ID (Pool-Plate-rowcolumn) Base pair change Homo/heterozygous Type of mutation Amino acid change Position of amino acid* SIFT score PSSM score
COMT1_A 1-4-G6 C>T Hetero Mis-sense Thr>Met 90 0.14 0
  2-1-E6 G>A Hetero Silent - 91 - -
  4-1-A3 G>A Homo Mis-sense Ala>Lys 95 0.36 +1
  4-1-A11 G>A Hetero Mis-sense Glu>Lys 92 0.24 +1
  5-7-D6 G>A Hetero Mis-sense Ala>Thr 65 0.25 +4
  6-10-G8 G>A Homo Mis-sense Glu>Lys 116 0.23 +1
COMT1_B 1-2-B8 C>T Hetero Mis-sense Ala>Val 275 0.45 +4
  3-3-E4 G>A Hetero Silent - 298 - -
  4-3-B10 G>A Hetero Mis-sense Met>Ile 261 0.55 0
  4-4-H5 G>A Homo Mis-sense Glu>Lys 271 0.41 −1
  5-8-A1 G>A Hetero Mis-sense Glu>Met 298 0.16 −4
  6-10-F3 C>T Hetero Mis-sense Pro>Ser 248 0.42 +2
HCT2_A 1-3-B12 G>A Hetero Mis-sense Gly>Arg 131 0.25 −2
  2-5-G6 G>A Hetero Silent - 100 - -
  5-8-C3 C>T Homo Silent - 72 - -
  5-6-C11 G>A Hetero Silent - 85 - -
HCT2_B 1-1-A4 G>A Hetero Mis-sense Glu>Lys 208 0.45 +1
  1-2-E7 G>A Homo Silent - 371 - -
  2-1-E12 C>T Hetero Silent - 395 - -
  2-6-H8 G>A Hetero Mis-sense Asp>Asn 330 0.56 −2
  3-2-G8 C>T Homo Silent - 359 - -
  3-2-F5 C>T Hetero Mis-sense Thr>Met 314 0.19 −1
  4-2-A9 G>A Homo Mis-sense Ala>Thr 275 0.50 −3
  4-1-B1 C>T Hetero Silent - 308 - -
  4-3-G4 G>A Hetero Mis-sense Val>Ile 310 0.59 +2
  5-8-C8 C>T Hetero Mis-sense Leu>Phe 267 0.37 +2
  6-10-A8 G>A Hetero Silent - 287 - -
  6-10-F11 G>A Hetero Silent - 414 - -
4CL1_B 2-1-H5 G>A Hetero Mis-sense Met>Ile 405 0.31 −3
  3-4-C10 C>T Hetero Mis-sense Thr>Met 345 0.11 −3
  4-1-E2 G>A Hetero Silent - 340 - -
  5-8-A10 G>A Homo Mis-sense Gly>Asp 370 0.28 −7
  6-9-G10 G>A Hetero Non-sense Trp>Stop 338 - -
4CL1_C 1-2-D3 C>T Hetero Intronic - - - -
  1-1-A7 G>A Hetero Intronic - - - -
  3-4-B7 C>T Hetero Intronic - - - -
  4-3-B6 C>T Hetero Intronic - - - -
  5-7-E8 G>A Homo Mis-sense Val>Ile 476 0.27 +1
  5-6-B8 C>T Hetero Silent Gly 485 - -
  6-9-H8 C>T Hetero Silent Gly 534 - -
W1 1-2-C5 G>A Hetero Mis-sense Gly>Ser 95 0.20 −4
  1-1-D7 C>T Hetero Mis-sense Ala>Val 58 0.19 Not found**
  2-1-H5 C>T Hetero Silent - 104 - -
  2-5-E5 C>T Hetero Intronic - - - -
  3-3-B3 G>A Homo Mis-sense Gly>Glu 101 0.17 −1
  3-3-E3 C>T Hetero Intronic - - - -
  3-2-G3 C>T Hetero Silent - 60 - -
  4-3-B5 G>A Homo Silent - 51 - -
  4-4-F9 G>A Hetero Intronic - - - -
  4-4-G9 G>A Hetero Mis-sense Arg>His 153 0.09 +1
  5-8-E6 C>T Homo Intronic - - - -
  6-10-F1 C>T Hetero Intronic - - - -
  6-9-H7 G>A Hetero Mis-sense Glu>Lys 143 0.14 −1
DOS 1-4-D8 G>A Hetero Silent - 253 - -
  1-2-G4 G>A Hetero Mis-sense Glu>Lys 328 0.12 −2
  2-5-D6 G>A Hetero Intronic - - - -
  2-6-G4 G>A Homo Intronic - - - -
  3-2-D1 G>A Homo Silent - 359 - -
  3-3-G9 G>A Hetero Mis-sense Val>Met 254 0.10 −6
  3-4-H2 C>T Hetero Mis-sense Ala>Val 305 0.13 −3
  4-3-F4 G>A Homo Intronic - - - -
  4-1-H2 G>A Hetero Silent - 294 - -
  5-7-A8 C>T Hetero Mis-sense Ala>Val 399 0.11 +2
  5-7-D1 G>A Hetero Silent - 315 - -
  5-5-H1 C>T Hetero Mis-sense Ala>Val 252 0.10 +2
  6-10-E6 G>A Hetero Silent - 277 - -
  6-9-F6 C>T Homo Silent - 346 - -
  1. *Position of amino acid acid change in rice protein, where wheat reference not available.
  2. **PSSM not found, compositionally biased region, not used in domain database search.