Microarray-based expression analysis of CAN1 and CAN2 genes at different developmental stages of Arabidopsis thaliana.Microarray expression data were retrieved from the Genevestigator database and processed as described in the Methods section. Numbers in brackets refer to the experiment ID from Genevestigator. Expression levels of genes encoding CAN1 [TAIR:At3g56170] and CAN2 [TAIR:At2g40410] proteins are displayed as signal values (y-axis) calculated for both genes in a given experiment. The x-axis indicates treatment conditions as described in the text and below. Light gray bars – controls, dark gray bars – expression in response to treatments within a given experiment. (A-C) Three examples of experiments demonstrating predominant expression of the CAN1 gene in Arabidopsis stem. (D-E) Two experiments showing the dependence of CAN1 expression on the stem growth phase. The abbreviations shown on the x-axis indicate the parts of stem: (tip) – stem tip, (up.mi.) - upper middle part of stem, (lo.mi.)- lower middle part of stem, (base) - stem base. (cov) - A. thaliana cov mutant affecting the vascular tissue development. (F-G) Correlation of CAN1 expression with xylem development in the root vascular bundle (F) and in cells induced to differentiate into tracheary elements (G). The abbreviations (2d-10d) indicate the number of days after culture induction. (H-I) correlation of CAN1 expression level with age-dependent (H) and sugar treatment induced senescence (I). The abbreviations (low/high N) and (gluc +/−) indicate the nitrogen and glucose concentration, respectively, in the culture medium. Expression profiles of senescence-associated BFN1 nuclease are also shown.