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Table 1 Transmission of p5cs2 mutant alleles by self-pollination and in reciprocal crosses with wildtype plants

From: Requirement of proline synthesis during Arabidopsis reproductive development

Genotype pollen donor1 Genotype pollen recipient1 Treatment Offspring with p5cs2allele [% ±SD2] Expected frequency3[%] Seedlings analysed
A/a1 A/a1 none 58.9 ±1.3* 66.7 474
A/a1 A/a1 50 mM NaCl 64.1 ±0.5 66.7 457
A/a2 A/a2 none 59.1* 66.7 164
A/a2 A/a2 50 mM NaCl 68.2 66.7 176
A/A A/a1 none 46.4 50 489
A/A A/a2 none 40.8* 50 309
A/a1 A/A none 46.1 50 371
A/a2 A/A none 47.7 50 461
  1. Transmission of the p5cs2 mutant alleles was determined by sulfadiazine (p5cs2-1) or BASTA (p5cs2-2) resistance of the progeny after selfing (first four rows) or reciprocal crossing of heterozygous p5cs2 mutants with wildtype plants.
  2. 1 A: P5CS2 wildtype allele; a1: p5cs2-1 mutant allele; a2: p5cs2-2 mutant allele.
  3. 2 SD was calculated from three batches of seeds from individual parent plants.
  4. 3 The expected frequency takes into account that homozygous p5cs2/p5cs2 seeds are usually non-viable in the presence of wildtype or heterozygous seeds in the same silique (compare Additional file 2 FigureS2).
  5. * indicates significant difference from the expected value (p<0.05 by Χ2 test with 1 degree of freedom).