|
Hordein primers
|
DNA concentration (amol/μl)
|
|---|
| |
Added DNA*
|
Calculated from Ctvalues
|
|---|
| | |
Pooled all (15): B1 (5), B3 (4), C (1), D (2), γ1 (2) and γ3 (1)
|
|---|
|
Common B
|
5.16
|
5.71 ± 0.18
|
|
B1
|
2.94
|
2.92 ± 0.11
|
|
B1a
|
2.94
|
2.74 ± 0.55
|
|
JQ867084
|
0.63
|
0.54 ± 0.14
|
|
B3
|
2.21
|
2.41 ± 0.61
|
|
B3a
|
2.21
|
1.81 ± 0.11
|
|
B3b
|
0.88
|
1.09 ± 0.02
|
|
Common C
|
0.53
|
0.51 ± 0.02
|
|
Common D
|
0.43
|
0.51 ± 0.01
|
|
Common γ1
|
1.04
|
0.97 ± 0.07
|
|
Common γ3
|
0.54
|
0.56 ± 0.00
|
|
JQ867080
|
0.54
|
0.47 ± 0.07
|
- *DNA concentration was measured for individual clones and the numbers represent amol/μl DNA in the pool.
- Note: the calculation based on three replicates and presented as means ± SD.
- B1-hordein clones: JQ859916, JQ859917, JQ867073, JQ867074, JQ867084; B3-hordein clones: JQ867085, JQ867086, JQ867087, JQ867089; C-hordein clone: JQ867090; D-hordein clones: JQ867076, JQ867077; γ1-hordein clones: JQ867078, JQ867079; γ3-hordein clone: JQ867080.
