OsABP
|
1st PCR
|
5′-ATCAAAATCATATCCCCTCCGC-3′
|
258 bp
|
Nested PCR
|
5′-TCCTCCCTCCATACGAAAATTCTG-3′
|
144 bp
|
OsDBH
|
1st PCR
|
5′-GAAAAGGGGAACAGTGCTAG-3′
|
150 bp
|
Nested PCR
|
5′-AACCAGTTCAGCTTTGGCTGCT-3′
|
100 bp
|
OsDSHCT
|
1st PCR
|
5′-GGCAGGTGGCAGGAAGATTATA-3′
|
490 bp
|
Nested PCR
|
5′-ACATCGCCGGTCATCAATCCGACAT-3′
|
310 bp
|
QRT-PCR for gene amplification
|
OsABP
|
Forward
|
5′-GGGGGAGGATGGTGAGTAAT-3′
|
211 bp
|
Reverse
|
5′-CCACCACCATCATCATCAAA-3′
|
OsDBH
|
Forward
|
5′-ACGATGGCACCTCAGAGAAT-3′
|
177 bp
|
Reverse
|
5′-TGGACCAAGTGACAGATGGA-3′
|
OsDSHCT
|
Forward
|
5′-GAGGAGGTGGAGAACACGAG-3′
|
237 bp
|
Reverse
|
5′-GTAGGCCTTGGCCATCTC-3′
|
α-Tubulin
|
Forward
|
5′-GGTGGAGGTGATGATGCTTT-3′
|
200 bp
|
Reverse
|
5′-ACCACGGGCAAAGTTGTTAG-3′
|
QRT-PCR for miRNA amplification
b
|
osa-MIR414
|
Forward
|
5′-TCATCCTCATCATCATCGTCC-3′
|
100 bp
|
osa-MIR164e
|
Forward
|
5′-GGGAGTTCTGTGATTGGAGAG-3′
|
100 bp
|
osa-MIR408
|
Forward
|
5′-TGGAGAAGCAGGGCACGTGA-3′
|
100 bp
|
U6
|
Forward
|
5′-CGATAAAATTGGAACGATACAGA-3′
|
150 bp
|
Reverse
|
5′-ATTTGGACCATTTCTCGATTTGT-3′
|