Table 9 Primer list for the stem-loop RT-PCR and the real-time PCR and their efficiencies
From: Identification of novel microRNAs in Hevea brasiliensisand computational prediction of their targets
Precursor name | Forward primer | Reverse primer | Unique melting pick | PCR efficiency |
|---|---|---|---|---|
Hbpre-miR156 | TGGTGATGTTGTTGACAGAAGATAGAGAGC | GCACAAAGGAGTGAGATGCAGAGTCC | Yes | 1,790 |
Hbpre-mir159a | GGTTAAGAAGTGGAGCTCCTTGAAGTC | GCTCCCTTCAATCCAAACAAGGATC | Yes | 1,958 |
Hbpre-mir159b | GTGGAGCTCCTTGAAGTCCAATAGAGG | AGAGCTCCCTTCAATCCAAACAAGG | Yes | 1,881 |
Hbpre-mir166b | GGGGAATGTTGTCTGGTTCGATG | TCAAATCAAACCCTGTTGGGGG | Yes | 1,738 |
Hbpre-mir396 | TGACCCTCTTCGTATTCTTCCACAGC | CCCACAGCTTTATTGAACCGCAAC | Yes | 1,782 |
Hbpre-mir408b | GACATACAAAGACTGGGAACAGGCAG | GCCACAAGCCAGGGAAGAGGC | Yes | 1,792 |
Hbpre-mir476 | GCCTTGTATGTTTCATTTAGTAATCCTTCT | GATAATCCTTCTATGCAAAGTCTTTTATGC | Yes | 1,732 |
Hbpre-mir2910 | CAGGTCCAGACATAGTAAGGATTGACAG | CGTTAACGGATTAACCAGACAAATCGC | Yes | 1,728 |
Hbpre-miRn1 | ACCAGGAACTGGTATCAACCCAGC | TGCTACCAATGAATCGGACCCACC | Yes | 1,837 |
Hbpre-miRn2 | CAGTAAATAGCAGTATCGTGGATAGGG | GTCCAATCATTGATCCTGAAAATTTCTAC | Yes | 1,828 |
Hbpre-miRn3 | TGGATTGGAGCCCAATACTGTGAC | CTGCTCCATTGATTTTACCATCTATGC | Yes | 1,873 |
Hbpre-miRn4 | TGCGTGGGTAGATTGAGCTGC | GCTCCTGAGATATGCAAGCCACAAG | Yes | 1,863 |
Hbpre-miRn6 | ACCTAGGATGTAGAAGAGCATAAC | ACTACATGAGTGGATATATAGGAATCC | Yes | 1,787 |
Hbpre-miRn7 | ATCTGCTACTCGGATAACCGTAG | GCAGCAAATCTTCCATAGCATCC | Yes | 1,925 |
Hbpre-miRn8 | TTTCTTGATTCTAGTGGGTGGTG | GGAATTAACCAGACAAATCGCTC | Yes | 1,905 |
Hbpre-miRn9 | AGTCGGGGGCATTCGTATTTC | GCAAATACTTTCGCAGTTGTTCG | Yes | 1,956 |
Hbpre-miRn10 | TGGATTTATGAAAGACGAACAACTGCG | TTCGAGCCCCCCAATTTCG | Yes | 1,712 |