Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness

Table 2 Progress of the rice transformation with transgenes encoding BP100 derivatives

BP100 derived peptide	Hygromycin resistant calluses*	Regenerated plants*	Acclimated plants*	Plants producing seeds*	Overall efficiency of the process compared to control plasmid
control	110**	80%	100%	100%
BP100.1	43 (39%)	21 (61%)	10 (48%)	9 (90%)	10%
BP100.2	23 (21%)	0 (0%)			<1%
BP100.2i	82 (75%)	20 (30%)	5 (25%)	2 (40%)	2%
BP100.3	20 (18%)	0 (0%)			<1%
BP100.2mi	83 (75%)	8 (12%)	8 (100%)	3 (38%)	3%

Absolute numbers and percentages relative to transformation with the control plasmid are given. Control transformation data is given in absolute numbers of hygromycin resistant calluses and percentages of transgenic plants, always relative to the previous step.
*A total of 1200 calluses were transformed with each plasmid in three independent experiments, each including transformation with the control vector. Only hygromycin resistant calluses and plants from different calluses (i.e. independent transformation events) were recorded.
**Only 15 of the 110 control hygromycin resistant calluses were further processed.

ISSN: 1471-2229