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Table 1 Kinetic parameters of CYP98A22 determined with p- coumaroyl quinate and p -coumaroyl shikimate as substrate

From: CYP98A22, a phenolic ester 3’-hydroxylase specialized in the synthesis of chlorogenic acid, as a new tool for enhancing the furanocoumarin concentration in Ruta graveolens

Substrate Product V max K m V i / K m
   (pmol.min-1.g-1FW) (μM) (μM.pmol-1.min-1.g-1FW)
p-coumaroyl quinate caffeoyl quinate 0.49 +/- 0.16 7 +/- 5 0.07 +/- 0.02
p-coumaroyl shikimate caffeoyl shikimate 0.19 +/- 0.03 24 +/- 7 0.008 +/- 0.002
  1. Reactions were carried out at 28°C during 30 min in a KPi buffer 20Mm pH 7.0 as described in material and methods. Reactions were stopped with addition of 0.1% trifluoroacetic acid. Data obtained were fitted with the Michaelis-Menten equation.