From: CYP98A22, a phenolic ester 3’-hydroxylase specialized in the synthesis of chlorogenic acid, as a new tool for enhancing the furanocoumarin concentration in Ruta graveolens
Substrate
Product
V max
K m
V i / K m
(pmol.min-1.g-1FW)
(μM)
(μM.pmol-1.min-1.g-1FW)
p-coumaroyl quinate
caffeoyl quinate
0.49 +/- 0.16
7 +/- 5
0.07 +/- 0.02
p-coumaroyl shikimate
caffeoyl shikimate
0.19 +/- 0.03
24 +/- 7
0.008 +/- 0.002