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Table 3 Targets for peach-specific miRNAs identified by degradome sequencing

From: Unique expression, processing regulation, and regulatory network of peach (Prunus persica) miRNAs

miRNA

Target genea

Align scoreb

Normalized reads at cleavage site (tpb)c

Categoryd

Target gene annotation

miRC2

ppa010261m

5

751.0

2

Zinc finger protein

miRC3

ppb024266m

3.5

350.5

2

NBS-LRR class disease resistance protein

miRC4

ppa012465m

4.5

10263.4

2

Unknown protein

miRC5

ppb024266m

5

350.5

2

NBS-LRR class disease resistance protein

miRC6a

ppa019098m

4

40.1

2

Pentatricopeptide (PPR) repeat-containing protein

miRC6a

ppa020475m

1

5997.8

0

Pentatricopeptide (PPR) repeat-containing protein

miRC6a

ppa023796m

5

15.0

4

Pentatricopeptide (PPR) repeat-containing protein

miRC6b

ppa019098m

4.5

40.1

2

Pentatricopeptide (PPR) repeat-containing protein

miRC6b

ppa020475m

1.5

5997.8

0

Pentatricopeptide (PPR) repeat-containing protein

miRC12

ppa000294m

5

600.8

2

Protein kinase family protein

miRC12

ppa000823m

5

851.1

2

Translation initiation factor

miRC13

ppa008890m

5

2353.1

2

Esterase/lipase/thioesterase family protein

miRC13

ppa010397m

4.5

100.1

3

Allene-oxide cyclase

miRC16

ppa018545m

5

100.1

3

FAR1-related sequence 3; zinc ion binding

miRC16

ppa022612m

5

100.1

3

FAR1-related sequence 3; zinc ion binding

miRC29

ppa002618m

5

50.1

3

RNA binding / translation initiation factor

miRC29

ppa002620m

5

50.1

3

RNA binding / translation initiation factor

miRC30

ppa004763m

4.5

25.0

4

Catalase

miRC45

ppa002666m

5

1552.0

2

Vernalization independence; DNA binding

  1. a Target gene is the identified transcript from the peach transcriptome (http://www.rosaceae.org/node/35).
  2. b Align score is calculated according to [7].
  3. c tpb, transcripts per billion, according to [21].
  4. d Category 0: >1 raw read at the position, abundance at position is equal to the maximum on the transcript, and there is only one maximum on the transcript; Category 1: >1 raw read at the position, abundance at position is equal to the maximum on the transcript, and there is more than one maximum position on the transcript; Category 2: >1 raw read at the position, abundance at position is less than the maximum but higher than the median for the transcript; Category 3: >1 raw read at the position, abundance at position is equal to or less than the median for the transcript; Category 4: Only 1 raw read at the position.