Figure 5

Three MiRNAs target 49 peach MYBs. (a) It was found that 49 MYBs were targeted by peach miR159, miR828 and miR858, some of which were targeted by more than one of the miRNAs. MiR858 targeted the majority of these MYBs. (b) Genomic organization of R2R3 MYB genes, location of target sites of miR159, miR828 and miR858, as well as a potential siRNA generation region. The highly conserved sequences are denoted by black area while the diverged sequence by gray box along the MYB coding regions. (c) Degradome confirmation of miR828 cleavage in three MYB transcripts. The red diamond and arrow indicate the cleavage site. (d) The tissue-specific accumulation of the phased siRNAs produced from the miR828-cleaved MYB transcripts is shown, as illustrated in Figure 4. (e) Phylogenetic analysis of functional relationship between miRNA-targeted peach R2R3 MYBs and the characterized Arabidopsis R2R3 MYBs according to previously work [39, 40]. MYB genes targeted by specific miRNA are differentiated by the same colors, as illustrated in (a).