Figure 4

Two peach trans-acting siRNA biogenesis pathways. (a) The miR390-TAS3 biogenesis pathway, showing the dual miR390 target sites on the PpTAS3 transcript as denoted by red arrows. The number of sRNA sequences mapped along the PpTAS3 transcript is plotted for sense (black line) and antisense (red line) strands, with the position of two conserved tasiARFs indicated below. The phasing radial graph is represented next to this. Each spoke of the radial graph represents 1 of the 21 phasing registers, with the total number of sRNAs mapping to that register plotted as distance from the center. Grey dots indicate the specific registers predicted by 21-nt processing from the 5’ and 3’ cleavage sites. The tissue-specific accumulation of the phased siRNAs in fruit, flower, leaf and root is shown below the phasing graph. (b) The miR828-TAS4 biogenesis pathway, showing the miR828 cleavage site on the PpTAS4 transcript as denoted with a red arrow at the 5' end. The phasing graph and tissue-specific accumulation of the phased siRNAs are shown, as illustrated in (a). (c) Degradome confirmation of the cleavage of two peach ARF transcripts by TAS3-derived tasiARFs using t-plot with the red diamond and arrow indicating cleavage sites.