Figure 6
The protein and mRNA levels of 1Bx14 in the grains of BSMV:1Bx14 infected spikes. Grain samples were collected at 20dpi from each wheat cv. infected with BSMV:1Bx14 or BSMV:00, and each of the grains was divided into two parts, one used for Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and the other for quantitative real-time PCR (qRT-PCR) analysis. (A) and (B) SDS-PAGE of HMW-GSs in the grain from cultivars Xiaoyan 6 or Shaanyou 225 inoculated with BSMV:00 or BSMV:1Bx14, respectively. Arrows indicate the reduced HMW-GS 1Bx14. (C) and (D) The scanning profiles of SDS-PAGE maps shown in Figure 6 and 6B. (E) and (F) QRT-PCR analysis of the relative expression of 1Bx14 in the same grain as in Figure 6A or 6B.