Figure 7

Expression profiles of grapevine DHN1 and DHN2 following treatment with various hormones. Total RNA was extracted from the leaves of V. vinifera sprayed with 50 μM MeJA, 100 μM SA and 100 μM ABA (A, B). Samples were taken at the indicated times, with time zero samples harvested immediately prior to spraying. Normalized transcript levels of DHN1 and DHN2 were determined by real-time qRT-PCR analysis, with the actin1 gene serving as an internal control. Each point represents the mean relative fold-change compared to baseline levels of expression from three experiments, while bars indicate standard deviations. Baseline expression levels were those measured just prior to treatment at time = 0. Asterisks (*), number signs (#) and reference marks (※) indicate significant differences (p < 0.05) in expression levels of target transcripts from V. vinifera treated with ABA, SA and MeJA, respectively, compared to the mock-treated controls.