Figure 5

Expression pattern of CCS52A2 in Arabidopsis . (A) to (F), CCS52A2p::CDS::GUS expression in shoot meristems (A) (B), root tips (C), lateral root (D), petals (E) and mature pollen (F) in 8-day-old seedlings. Bar = 100 μm. (G), mRNA expression results of CCS52A2, CLV3 and WUS in SAM of 10-day-old seedling (WT and ccs52a2-3) from real time-PCR. (H) to (O), CCS52A2 expressions in SAMs of inflorescence shoot (H) and 8-day-old seedling (J) and pollen (L) (M) (N) by using in situ hybridization. (I) (K) (O) Hybridization with sense probes as a control. Bar = 50 μm. (P), CCS52A2 expression in various Arabidopsis tissues by RT-PCR. ACTIN2 is shown as a loading control. RT = Rossette leaves, ST = stem, CL = Cauline leaves, IF = Inflorescence, SL = Silique, RT = Root. (Q) and (R), CYCB1;1::GFP expression in roots of 8-day-old seedlings of WT (Q) and ccs52a2-3 (R). (S), mRNA expression results of CYCB1;1 in SAM of 10-day-old seedling (WT and ccs52a2-3) from real time-PCR.