Figure 6

Intercellular adhesion in the cortex tissue of ‘Royal Gala’ (RG) control (A, C, E) and transgenic PG1as-41 (B, D, F) fruit. Toluidine blue-stained sections of cell wall boundaries at harvest (A, B); and following ripening for 16 weeks at 5°C - stored (C, D). Immunolocalisation of a pectin epitope with a low degree of esterification in the cell walls of apple fruit after ripening for 16 weeks at 5°C visualised using the antibody JIM5 (E, F). Arrows indicate materials filling the space where two cells join (A, B, D, F). In (C) and (E), solid arrowheads indicate remnants of cell wall material protruding into the intercellular space. In (C) the open arrowhead indicates a region where material has been lost between adjacent cells. Bar = 10 μm.