Figure 5

Structural changes occurring in the exocarp and cortex of transgenic PG1as and ‘Royal Gala’ control fruit. Toluidine blue-stained sections from: ‘Royal Gala’ (RG) at harvest (A, B) and following ripening for 16 weeks at 5°C - stored (C, D); PG1as-41 at harvest (E, F) and following ripening for 16 weeks at 5°C - stored (G, H). Coloured lines show the approximate extent of the epidermis/cuticle (blue), hypodermal cell layers (red) and cortex (green). Note the thickening of the hypodermal layers observed in the control (C, D) does not occur in the suppressed PG1as-41 line (G, H). Scale bars = 100 μm (A, C, E, G) and 50 μm (B, D, F, H).