Level of mature miRNAs in the smRNA libraries vs in planta. A. Northern blot showing the level of mature miRNAs in whole caryopsis tissues harvested from three stages: 1–5 DPA (A), 6–10 DPA (B) and 11–15 DPA (C). The same amount of total RNA was loaded based on spectrophotometer readings. The intensity of each band was quantified using a phosphoimager and is indicated as a percentage. B. Distribution of three known miRNAs between the three stages (A, B, C) using read numbers in the three smRNA libraries (blue) and their abundance detected by northern blot in 5A (red). The percentages for each miRNA refer to the total abundance or reads of the miRNA detected across the three samples or libraries.