Figure 2
Figure 2 – Phylogenetic analysis of Salvia sclarea diterpene synthases. The phylogenetic tree was generated based on multiple amino acid sequence alignments (DIALIGN-TX), phylogenetic analysis (PhyML, four rate substitution categories, LG substitution model, BIONJ starting tree, 100 bootstrap repetitions, rooted with the outgroup copalyl diphosphate synthase/kaurene synthase from the moss Physcomitrella patens PpCPS/KS [BAF61135]), and visualization in treeview. Asterisks indicate nodes supported by > 90% bootstrap values. Protein abbreviations [NCBI GenBank accession no. ]: JsCPS/KS, Jungermannia subulata ent-copalyl diphosphate/ent-kaurene synthase [BAJ39816]; SmCPS/KS1, Selaginella moellendorfii labda-7,13E-dien-15-olsynthase [AEK75338]; TbTS, Taxus brevifolia taxadiene synthase [AAC49310]; GbLS, Ginkgo biloba levopimaradiene synthase [AAL09965]; AbCAS, Abies balsamea cis-abienol synthase [JN254808]; AgAS, Abies grandis abietadiene synthase [AAK83563]; PaLAS, Picea abies levopimaradiene/abietadiene synthase [AAS47691]; PaIso, Picea abies isopimaradiene synthase [AAS47690]; CmKS, Cucurbita maxima kaurene synthase [AAB39482]; AtKS, Arabidopsis thaliana ent-kaurene synthase [AF034774]; PtKS, Populus trichocarpa kaurene synthase [XM_002311250]; RcKS, Ricinus communis kaurene synthase-like [XP_002533694]; HaKS, Helianthus annuus kaurene synthase [CBL42917]; SdKS, Scoparia dulcis kaurene synthase-like [AEF33360]; OsKS1, Oryza sativa ent-kaurene synthase [AY347876]; OsKSL4, O. sativa syn-pimaradiene synthase [AY616862]; OsKSL8, O. sativa syn-stemarene synthase [AB118056]; OsKSL10, O. sativa ent-pimaradiene synthase [DQ823355]; RcKSL1, R. communis kaurene synthase-like [XM_002525795]; RcKSL2, R. communis kaurene synthase-like [XM_002525790]; RcKSL3, R. communis kaurene synthase-like [XM_002525796]; SmKSL, Salvia miltiorhizza kaurene synthase-like [EF635966]; NtKSL, N. tabacum kaurene synthase-like [CCD33019]; SlPHE, Solanum lycopersicum phellandrene synthase [FJ797957]; ShSBS, Solanum habrochaites bergamotene/santalene synthase [FJ194970]; CcCLS, Cistus creticus copal-8-ol synthase [DJ93862]; NtCPSL, Nicotiana tabacum 8-hydroxy copalyl diphosphate synthase [CCD33018]; SmCPSL, S. miltiorhizza copalyl diphosphate synthase-like [EU003997]; HaCPS, H. annuus copalyl diphosphate synthase [CBL42915]; AtCPSL, A. thaliana ent-copalyl diphosphate synthase [AAA53632]; CmCPS, C. maxima copalyl diphosphate synthase [AF049905]; SdCPSL, S. dulcis copalyl diphosphate synthase-like [AB046689]; SlCPSL, S.lycopersicum copalyl diphosphate synthase-like [AB015075]; ZmCPSL, Zea mays ent-copalyl diphosphate synthase [AY562491]; OsCPS1, O. sativa ent-copalyl diphosphate synthase [Q6ET36]; OsCPS2, O. sativa ent-copalyl diphosphate synthase [Q6Z5I0]; OsCPS4, O. sativa syn-copalyl diphosphate synthase [Q6E7D7].