Figure 7
Functional characterization of TaeIF5A1 using the S. cerevisiae expression system. A. RNA gel blot analysis of the expression of exogenous TaeIF5A1 in yeast at different induction times. 1–2, empty pYES2 transformants were cultured in induction medium (SC-Ura-/Gal 2%) or suppression medium (SC-Ura-/Glu 2%) at 30°C for 36 h. 3–8, yeast transformants harboring TaeIF5A1 were cultured in induction medium at 30°C for 0, 12, 24, 36, 48 and 60 h. B. Abiotic stress tolerance analyses of TaeIF5A1. Comparison of growth between two transgenic yeast lines (harboring empty pYES2 or pYES2-TaeIF5A1). Growth was detected by measuring the absorbance at 600 nm after culturing at 30°C for 24 h in liquid medium supplemented with different concentrations of NaCl, KCl, LiCl or sorbitol. C. Investigation of the effect of TaeIF5A1 on protein synthesis. Yeast transformants harboring TaeIF5A1, TaPrx1 (positive control), or empty pYES2 were cultured in induction medium at 30°C for 0, 12, 24, 36 and 48 h for soluble protein content analysis. * means significant difference (P < 0.05) between yeast transformants harboring TaeIF5A1 and TaPrx1.