Organelle dynamics within a single areole of a stage 2 or window stage lace plant leaf. (A) Half of a single areole of a window stage leaf. Note that the image is sectioned into three stages, the right most section being NPCD, the middle being EPCD and the left most being LPCD. (B) Perinuclear accumulation of chloroplasts. (C) Mitochondrial dynamics over the gradient of PCD, visualized via CMXRos. Note that this gradient corresponds with that seen in panel A. NPCD stage cells contain many individual mitochondria in the cytosol. EPCD stage cells contain mitochondrial aggregates (arrows), along with some individual mitochondria. LPCD stage cells depict either aggregates or mitochondria, or a lack of staining, indicating the loss of ΔΨm (mitochondrial membrane potential). (D) Gradient of TUNEL positivity, indicating the cleavage of DNA by endonucleases throughout PCD in the lace plant. Note that this gradient corresponds with that in panel A. NPCD and EPCD stage cells do not contain TUNEL positive nuclei, indicating intact nuclear DNA. LPCD stage cells do contain TUNEL positive nuclei, signifying DNA cleavage by endonucleases. Scale bars: A= 100μm, B= 20μm, C-D= 120μm.