Table 1 Forward and reverse primers used in real-time PCR and RT-PCR analyses
From: Apple skin patterning is associated with differential expression of MYB10
Gene identifier (Genbank) | Name | Forward primer | Reverse primer |
|---|---|---|---|
CN938023 | Actin | TGACCGAATGAGCAAGGAAATTACT | TACTCAGCTTTGGCAATCCACATC |
CN944824 | CHS | GGAGACAACTGGAGAAGGACTGGAA | CGACATTGATACTGGTGTCTTCA |
CN946541 | CHI | GGGATAACCTCGCGGCCAAA | GCATCCATGCCGGAAGCTACAA |
CN491664 | F3H | TGGAAGCTTGTGAGGACTGGGGT | CTCCTCCGATGGCAAATCAAAGA |
AF117268 | DFR1 | GATAGGGTTTGAGTTCAAGTA | TCTCCTCAGCAGCCTCAGTTTTCT |
AF117269 | LDOX | CCAAGTGAAGCGGGTTGTGCT | CAAAGCAGGCGGACAGGAGTAGC |
AF117267 | UFGT | CCACCGCCCTTCCAAACACTCT | CACCCTTATGTTACGCGGCATGT |
DQ267896 | MYB10 | TGCCTGGACTCGAGAGGAAGACA | CCTGTTTCCCAAAAGCCTGTGAA |
CO867070 | MYB17 | TGGCTCCAGAAAAGCAAATCA | GGCCGCTTGCAGAATCTGTA |
CN934367 | bHLH3 | AGGGTTCCAGAAGACCACGCCT | TTGGATGTGGAGTGCTCGGAGA |
DQ266451 | bHLH33 | ATGTTTTTGCGACGGAGAGAGCA | TAGGCGAGTGAACACCATACATTAAAGG |
DQ886414 | MYB10 cDNA | GCGGTACCGGTAGCAGGCAAAAGAATAGCTAAGC | GCGGATCCCACATTTACAAGCAAGGAAAATA |
AY603367 | TRIM | CGGGATGTGACAATTTGGTA | GCGATGTGGGATGTTACAAT |
EU518249 | MYB10 -2029 to -1229 | GAAATCGTTCGAAGGTCTAAGG | TTCGTTGGATTCCGTTAAGC |
EU518249 | MYB10 -1411 to -678 | AACCTTCACAAGGGTTGTCG | AATGGATGGAATGGAACGAA |
EU518249 | MYB10 -677 to 47 | TTCGTTCCATTCCATCCATT | AGTCCAGGCACCTTTTCTCA |
EU518249 | MYB10 -1007 to -684 | TGGAGTTAAATTAAYAAGGY | ARARRARAAAATCCTARCCC |
EU518249 | MYB10 -534 to -184 | GAATGAAGAAGAGGGAAAAAAA | ATCCACARAARCAAACACTRACA |