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Table 2 Effect of different co-cultivation factors on transformation efficiency using established embryogenic callus cultures

From: Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

Co-cultivation factors Treatments No. of co-cultivated calli No. of transformed calli Transformation efficiency (%)
Agrobacterium conc. (OD600) 1 (OD600 = 0.6) 80 10 12.5 ± 11.1
  1/10 102 2 1.9 ± 4.1
Co-cultivation temperature (°C) 20 215 10 4.7 ± 4.3
  27 350 2 0.6 ± 1.2
  1. *The transformation efficiency was estimated by the proportion (p) of transformed calli (p = x/n), where x was the number of transformed calli and n the number of co-cultivated calli. A 3 δ confidence limit for binomial distribution was calculated using the formula with a level of confidence of 99%