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Table 5 The expression levels of CaCc and CaCe isoforms in leaves of eight-year-old C. arabica.

From: RBCS1 expression in coffee: Coffea orthologs, Coffea arabica homeologs, and expression variability between genotypes and under drought stress

  WT Y RQ Cc RQ Ce RQ RBCS1-T Cc/Ce
   U1-08 21.65 6.22 27.87 3.48
  I WS-08 6.95 8.99 15.94 0.77
I59   U2-08 35.17 6.21 41.38 5.66
   U1-08 20.56 5.46 26.03 3.76
  NI WS-08 11.74 1.60 13.34 7.33
   U2-08 24.19 9.35 33.54 2.59
   U1-08 - 11.58 11.58 -
  I WS-08 - 10.38 10.38 -
Rubi   U2-08 - 8.00 8.00 -
   U1-08 - 15.99 15.99 -
  NI WS-08 - 9.52 9.52 -
   U2-08 - 12.46 12.46 -
  WT Y RQ Cc RQ Ce RQ RBCS1-T Cc / Ce
   U1-08 14.62 7.18 21.80 2.03
  I WS-08 19.55 6.04 25.59 3.23
I59   U2-08 25.90 11.81 37.72 2.19
  NI U1-08 35.32 8.16 43.48 4.33
   WS-08 17.68 6.12 23.80 2.89
   U2-08 19.62 6.65 26.26 2.95
   U1-08 - 7.21 7.21 -
  I WS-08 - 8.30 8.30 -
Rubi   U2-08 - 8.79 8.79 -
   U1-08 - 14.05 14.05 -
  NI WS-08 - 7.16 7.16 -
   U2-08 - 8.12 8.12 -
  1. Leaves of the cultivars I59 and Rubi were harvested in daytime (top: between 10:00 and noon) or night time (bottom: between 3:00 and 5:00 am). The points of analysis were before (U1, unstressed), during (WS, water stress) and after (U2, unstressed) the dry season of 2008. Corresponding predawn leaf water potentials (Ψpd) are given in the Table 3. The results of the relative quantification (RQ) are given for the cultivars IAPAR59 (I59) and Rubi grown in the field with (I) or without (NI) irrigation during the dry season (WT: water treatment). RBCS1 targets corresponded to CaCe (Ce) and CaCc (Cc) amplified by the E18244 and C18244 primer pairs (Table 1), respectively. Total RBCS1 expression (RQ RBCS1-T ) corresponded to RQ Cc + RQ Ce , while Cc/Ce ratios corresponded to the RQ Cc /RQ Ce ratios. For the Rubi cultivar, CaCc gene expression was not detected (-). Results were normalised using the expression of the GAPDH reference gene.